Description
This Human WAP Four-Disulfide Core Domain Protein 2 (HE4) ELISA Kit is intended for quantitative detection of human HE4 in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk. Strip well format. Reagents for up to 96 tests.
This human HE4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for HE4 has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: E31-F124) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for HE4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HE4 amount of sample captured in plate.
The capture antibody is a polyclonal antibody from sheep, the detection antibody is a biotinylated polyclonal antibody from sheep. Expression system for standard: WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation